Livestock Diagnostics
Pregnancy Rapid Test
Optimize reproductive efficiency
TrillionsMED’s Milk Pregnancy Test helps maximize milk production and profitability. Find a milk testing laboratory and do more with your cow and goat milk samples
• Rapid & Visual
• Easy to use
• Application
Early pregnancy detection in bovine is an important part of the reproduction process. Using an accurate, fast, and easy pregnancy diagnosis method is the key to improve breeding efficiency and milk yield. TrillionsMed Bovine Pregnancy Test Kit is a blood pregnancy test for dairy cows based on double antibody sandwich technology It can be used to detect pregnancy-associated glycoprotein serum / plasma / whole blood (EDTA or heparin sodium) samples of cows after 28 days post-breeding.
Early Diagnosis
The test can be performed as early as 28 days post-breeding.
Quick & Reliable Results
Get test results in 5-20 min.
High Accuracy & Cost-effective
Low cost and high conformity compared to ultrasound.
Safe for Embryos
Safer than rectal palpation & causes less stress response than ultrasound device.
Simplified Procedures
No special device is needed, and the results can be interpreted visually.
Application
On-site test and laboratory testing for small, medium & large scale farm.
Bovine Pregnancy Test Methodolgies
- 5 commonly used cattle pregnancy detection methods
- Comparison of several detection methods
- Guidelines for the selection of assays
Rectal Palpation
Rectal palpation is the most traditional and widely used technique for detecting bovine pregnancy. But it can only be made after 45 to 60 days of mating, as the detection window period is long，and the immaturity of the technology is easy to cause embryo loss. Skilled breeding technicians are required.
Ultra-sound Diagnosis
There are many applications in pastures, but the accuracy of the test results is affected by the operator’s proficiency in ultrasound technology, the time of ultrasound detection after mating, and the ultra-sound instrument itself.
The application of Ultra-sound examination will cause certain mechanical damage, embryo loss, cross-infection, and epidemic prevention risks, and the one-time investment is large, and the maintenance cost in the later stage is high.
Blood test
By measuring progesterone (progesterone) in the blood of Bovine, pregnant cattle can be identified in the early stage of pregnancy and non pregnant cows can be identified.
Because the corpus luteum and embryos secrete a large amount of progesterone, the plasma progesterone of pregnant cattle at 21 days of mating was significantly higher than that of non-pregnant cattle (4.675 ng·mL-1 vs 0.995 ng·mL-1, P<0.01) and can be detected in milk and blood. Therefore, methods such as radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and colloidal gold immunoassay (GICA) were established to determine plasma or milk progesterone levels, and certain results were achieved. However, some studies have shown that 35%~44% of non-pregnant cows have a prolonged luteal phase without normal return to estrus, and there is no significant difference in plasma progesterone levels between pregnant cattle with prolonged luteal phase and normal pregnant cattle [18-19], which increases the false positive of progesterone test and affects the overall accuracy of pregnancy diagnosis.
Blood Test
Pregnant cattle are identified early in pregnancy by measuring the blood of the cattle for the presence of PAGS/PSPB ( pregnancy-specific protein B, ) and to identify unpregnant cattle.
Milk Test
By detecting glycoproteins (PAGs) in milk, 35 days after mating, open cows were identified.
These methods are based on immunological principles, which are not only highly accurate and free from human interference, but also avoid premature direct contact with the embryo and cause miscarriage.
It is necessary to have a laboratory，a microplate reader, an incubator and professional laboratory personnel.
Blood Test
By measuring PAGS/PSPB (Pregnancy Specific Protein B) in the blood of cattle, pregnant cattle can be identified in early pregnancy and unpregnant cattle can be identified.
These methods are based on immunological principles, which are not only highly accurate and free from human interference, but also avoid premature direct contact with the embryo and cause miscarriage.
There is no need for laboratories, microplate readers, incubators, and professional experiment
| Methodology | PAGS Blood Test | PAGS Milk Test | PAGS Blood Test |
Diagnostic Principles | Enzyme-Linked Immunosorbent Assay | Enzyme-Linked Immunosorbent Assay | Colloidal Gold |
| Test Target | PSPB | Specific protein PAG | Specific Protein PAG |
| Diagnostic Platform | Quantitative Testing | Quantitative Testing | Quantitative Testing |
| Sample type | Serum/Plasma | Milk | Whole Blood |
| Accuracy | ＞99.5% | 92-95%： Related to Mating Period | 95-96% |
| Pregnancy Test | High target | Relatively High | High Target |
| Confirm Pregnancy | 28 Days After Mating | Relatively High | 28 Days After Mating |
| Cross-Contamination Sampling | Low | High target | Low |
| Sample Interference | Less | More | Less |
| Human Interference Factors | Less | Less | Less |
PAGS Blood Test | VS | PAGS Milk Test | VS | PAGS Blood Test | |
Advantages | Quantitative determination, high detection rate Can be detected 28 days after breeding Re-testing further improves the detection rate | Blood sampling is not required | No instrument is required Can be detected 28 days after breeding Re-testing further improves the detection rate | ||
Disadvantages | Blood sampling and instrumentation are required | Testing can only be done 35 days after breeding. The detection rate is greatly affected by time Samples are easily contaminated | The accuracy is slightly lower than the ELISA method |
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Breeding Scale |
Features |
Preferred Method (PAGS) |
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Small ranches, Free-range farmers |
The breeding scale is small, there is no testing laboratory and supporting instruments, there is no relevant laboratory personnel, and the sensitivity of the test results is not high. |
Colloidal Gold (Blood test) |
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Small and Medium-sized pastures |
The breeding scale is between 200-1000 heads, there is a testing laboratory, but there is a lack of supporting instruments, or the level of operators is limited, and the sensitivity of the test results is required. |
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Large-Scale pastures |
The breeding scale is more than 1,000 heads, with testing laboratories and supporting instruments, and there are testing personnel with high sensitivity requirements. |
ELISA（Blood test） |
TrillionsMED Testing Solution
TrillionsMED Pregnancy Tests
TrillionsMED Milk Pregnancy Test
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TrillionsMED Rapid Visual Pregnancy Test
TrillionsMED Farm Pregnancy Test